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Functional expression of GABAA chloride channels and benzodiazepine binding sites in baculovirus infected insect
D B Carter1, D R Thomsen, W B Im
1Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
Bio/Technology (Nature Publishing Company)
|June 1, 1992
Abstract:
We have employed the baculovirus expression system for the production of insect cell membranes having GABA/benzodiazepine binding sites. Three recombinant baculoviruses each harboring a different GABAA receptor cDNA were introduced into insect cells by simultaneous infection. Infected cells expressed GABA responsive Cl- channels and benzodiazepine binding sites with the same pharmacological specificity as animal cells expressing these receptor subunits.