An inducible gene expression system for Neurospora crassa
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M E Collins1, G Briggs, C Sawyer
1Protein Engineering Department, AFRC Institute of Food Research, Shinfield, Reading, UK.
Enzyme and Microbial Technology
|May 1, 1991
Abstract:
Neurospora crassa acetyl CoA synthetase is highly induced when the growing mycelium is transferred from sucrose- to acetate-based medium. The inducible promoter of this gene has been isolated and used to control the expression of glutamate dehydrogenase. Transformants containing this expression cassette show gdh levels up to 25 times higher than the nontransformed host strain. This expression cassette will form the basis of a system of heterologous gene expression.