[Acetylcholine in the bovine choroid and the cholinergic effect of pilocarpine]

In this study the authors have compared the cholinergic tone in a collection of bovine choroids after instillation of pilocarpine in the conjunctival fornix in one group using the opposite choroid from the same animal as a control. The tests for acetylcholine (ACh) were assayed on guinea-pig ileum. The choroidal nerves in the pilocarpinised eyes had an average level of 0,50 mug of ACh, the homologous choroidal nerves of the controls had an average level of 0,45 mug of standard ACh per 150 mg of tissue. The pilocarpine had been instilled in the conjunctival fornix as a 3 per cent aqueous solution of pilocarpine HCl, ten times three drops in intervals of three minutes. One hour after the last application the animals were sacrificed and the eyes enucleated. The authors discuss the clinica importance of the infiltration of pilocarpine into the choroid.