Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[3H]zonisamide binding in rat brain.

T Mimaki1, Y Suzuki, T Tagawa

  • 1Department of Pediatrics, Osaka University Medical School, Japan.

Medical Journal of Osaka University
|March 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SU-E-I-59: Investigation of the Usefulness of a Standard Deviation and Mammary Gland Density as Indexes for Mammogram Classification.

Medical physics·2017
Same author

SU-E-I-62: Investigation of Dominant Factors Affecting Fatigue in Image Reading of Radiologists.

Medical physics·2017
Same author

Re: Anterior mediastinal solid tumours in adults: characterisation using dynamic contrast-enhanced MRI, diffusion-weighted MRI, and FDG-PET/CT. A reply.

Clinical radiology·2016
Same author

Anterior mediastinal solid tumours in adults: characterisation using dynamic contrast-enhanced MRI, diffusion-weighted MRI, and FDG-PET/CT.

Clinical radiology·2015
Same author

Low-dose CT screening using hybrid iterative reconstruction: confidence ratings of diagnoses of simulated lesions other than lung cancer.

The British journal of radiology·2015
Same author

Changes of the apparent diffusion coefficient in brain diffusion-weighted images due to subject positioning: A simulation study.

Journal of neuroradiology = Journal de neuroradiologie·2015

Zonisamide binds to specific sites in rat brain synaptosomes. These binding sites show a strong correlation with benzodiazepine receptors, suggesting a potential mechanism for zonisamide

Area of Science:

  • Neuropharmacology
  • Neurochemistry

Background:

  • Zonisamide is known to inhibit [3H]flunitrazepam and [3H]muscimol binding in rat brain.
  • Understanding the specific binding interactions of zonisamide is crucial for elucidating its pharmacological effects.

Purpose of the Study:

  • To characterize the specific binding of [3H]zonisamide in rat brain.
  • To investigate the relationship between zonisamide binding sites and known neurotransmitter receptors.

Main Methods:

  • Radioligand binding assays using [3H]zonisamide on crude synaptosomal fractions of rat brain.
  • Scatchard plot analysis to determine binding kinetics (Kd and Bmax).
  • Displacement studies with clonazepam and GABA to assess receptor interactions.

Main Results:

Related Experiment Videos

  • [3H]zonisamide exhibited saturable binding to rat brain synaptosomes.
  • Binding affinity (Kd) was 90 nM with a maximal binding capacity (Bmax) of 1.40 x 10(3) fmol/mg protein.
  • Clonazepam inhibited, while GABA enhanced, specific [3H]zonisamide binding.

Conclusions:

  • Specific binding sites for zonisamide were identified in the rat brain.
  • These binding sites demonstrate a significant correlation with benzodiazepine receptors.
  • The findings suggest that zonisamide may exert its effects through interactions with the benzodiazepine receptor complex.