Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surface presentation of protein epitopes using bacteriophage expression systems.

G P Smith1

  • 1University of Missouri, Columbia.

Current Opinion in Biotechnology
|October 1, 1991
PubMed
Summary

Filamentous phage display libraries allow screening for foreign peptides that bind to specific proteins. Future libraries will display diverse antibodies for broader applications in biotechnology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cutaneous manifestations of small fibre polyneuropathy.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Analysis of US Food and Drug Administration Warning Letters within dermatological care products.

Clinical and experimental dermatology·2021
Same author

Evaluation of malignancy risk in scleroderma by antibody profile.

The British journal of dermatology·2020
Same author

Analysis of the first 5 years of an interdisciplinary Rheumatology-Dermatology clinic.

Clinical and experimental dermatology·2020
Same author

Development of an auxotrophic, live-attenuated Brucella suis vaccine strain capable of expressing multimeric GnRH.

Vaccine·2019
Same author

Mycobacterium tuberculosis and whole-genome sequencing: how close are we to unleashing its full potential?

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2017

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Protein Engineering

Background:

  • Filamentous phage display is a powerful technique for discovering novel protein interactions.
  • Current phage libraries primarily display short, random peptides.
  • Screening involves affinity purification based on binding to target proteins like antibodies.

Purpose of the Study:

  • To summarize the capabilities of filamentous phage display libraries for screening binding proteins.
  • To highlight the ongoing development of more diverse phage display libraries.

Main Methods:

  • Construction of vast libraries of filamentous phage expression vectors.
  • Display of foreign polypeptides on the phage virion surface.
  • Screening via affinity purification of phage binding to target proteins.

Related Experiment Videos

Main Results:

  • Demonstrated success in screening for specific peptide-antibody interactions.
  • Identified limitations of current libraries displaying only short random peptides.

Conclusions:

  • Filamentous phage display is effective for identifying specific binding peptides.
  • Expansion to display diverse antibodies will broaden the scope of this technology.