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Lamina, a novel multicellular form of Methanosarcina mazei S-6
L E Mayerhofer1, A J Macario, E Conway de Macario
1Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany.
Abstract:
A novel multicellular form of Methanosarcina mazei S-6 is described. It was termed lamina, and it formed during the exponential growth phase when packets or single cells were grown in 40 mM trimethylamine and a total concentration of 8.3 to 15.6 mM Ca2+ and/or Mg2+, in cultures that were not shaken. A distinct molecular event represented by the increment in expression and a spatial redistribution of an antigen during lamina formation is documented.
Insights
Researchers discovered a new multicellular form of Methanosarcina mazei S-6, named lamina. This structure forms during exponential growth under specific nutrient and mineral conditions without shaking, involving a molecular event.
Area of Science:
- Microbiology
- Cell Biology
- Biochemistry
Background:
- Methanosarcina mazei S-6 is an archaeon with known metabolic capabilities.
- Multicellularity is observed in various microbial systems, influencing community structure and function.
- Understanding microbial development and morphology is crucial for ecological and biotechnological applications.
Purpose of the Study:
- To describe a novel multicellular form of Methanosarcina mazei S-6.
- To identify the conditions under which this multicellular form develops.
- To document molecular events associated with its formation.
Main Methods:
- Culturing of Methanosarcina mazei S-6 under controlled conditions.
- Microscopy to observe cell morphology and multicellular structures.
- Biochemical analysis to identify molecular changes during formation.
Main Results:
- A novel multicellular form, termed lamina, was observed in Methanosarcina mazei S-6.
- Lamina formation occurred during the exponential growth phase.
- Specific conditions favoring lamina formation include 40 mM trimethylamine and 8.3-15.6 mM Ca2+/Mg2+ in static cultures.
- A molecular event involving antigen expression and redistribution was documented during lamina formation.
Conclusions:
- Methanosarcina mazei S-6 can adopt a multicellular lifestyle under specific environmental cues.
- The formation of lamina represents a distinct developmental stage with associated molecular changes.
- This finding expands the understanding of microbial morphogenesis in archaea.