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Nucleotide sequence of the complementary DNA for human Pit-1/GHF-1
1Department of Laboratory Medicine, Osaka University Medical School, Japan.
Biochimica Et Biophysica Acta
|January 6, 1992
Summary
Researchers identified human cDNA clones for Pit-1/GHF-1, a pituitary-specific DNA binding factor. This protein shows high conservation across species, suggesting a vital role in pituitary development and function.
Area of Science:
- Molecular Biology
- Genetics
- Endocrinology
Background:
- Pit-1/GHF-1 is a crucial pituitary-specific transcription factor.
- Understanding its structure and conservation is key to pituitary development research.
Purpose of the Study:
- To isolate and characterize human cDNA clones encoding Pit-1/GHF-1.
- To analyze the structural features and conservation of human Pit-1/GHF-1.
Main Methods:
- Polymerase Chain Reaction (PCR) on human pituitary RNA.
- Reverse transcription to obtain cDNA.
- Sequence analysis of the obtained clones.
Main Results:
- Successfully obtained human cDNA clones for Pit-1/GHF-1.
- The human Pit-1/GHF-1 gene is approximately 1.3 kb, with specific non-coding and coding regions.
- The predicted peptide has 291 amino acids and is highly conserved across mammalian species (mouse, rat, bovine).
- The 5' non-coding region shows significant conservation with the rat sequence up to the transcription start site.
Conclusions:
- The high conservation of Pit-1/GHF-1 suggests a fundamental role in pituitary function across species.
- Characterization of the human Pit-1/GHF-1 cDNA provides a basis for further functional studies.