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Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA
G W Mulheron1, N L Bossert, J A Lapp
1Department of Obstetrics/Gynecology, Duke University Medical Center, Durham, NC 27710.
The Journal of Clinical Endocrinology and Metabolism
|February 1, 1992
Abstract:
Human granulosa-luteal cells and cumulus cells obtained from women undergoing in vitro fertilization and embryo transfer (IVF-ET) were examined for the presence of TGF-beta 1 and TGF-beta 2 mRNA by reverse transcription-polymerase chain reaction (RT-PCR) analysis. RT-PCR analysis revealed that both follicle cell types express mRNA for both TGF-beta subtypes. Verification of RT-PCR products was done by restriction enzyme digestion analysis. These results suggest a role(s) for TGF-beta 1 and TGF-beta 2 in the development of human granulosa-luteal cells and the oocyte-cumulus cell complex.