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CD58 and CD59 molecules exhibit potentializing effects in T cell adhesion and activation
M Deckert1, J Kubar, A Bernard
1Institut National de la Santé et de la Recherche Médicale INSERM U343, Faculté de Médecine, France.
Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1992
Summary
The study shows that CD58 and CD59 molecules on accessory cells synergistically enhance T cell adhesion and activation. These molecules play a crucial role in immune responses by promoting T cell interactions.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD58 and CD59 are cell surface molecules involved in immune regulation.
- Their specific roles in T cell adhesion and activation require further elucidation.
Purpose of the Study:
- To investigate the individual and combined effects of CD58 and CD59 on T cell adhesion and activation.
- To determine if these molecules exhibit additive or synergistic functions.
Main Methods:
- Generation of stable Chinese hamster ovary (CHO) cell transfectants expressing CD58, CD59, or both.
- Rosetting assay to measure T cell adhesion to CHO transfectants.
- Assay to measure T cell proliferation in response to CHO transfectants and submitogenic doses of PHA + rIL-1 alpha.
Main Results:
- CD59 alone mediated 12% rosette formation, while CD58 alone induced 29% rosette formation.
- Co-expression of CD58 and CD59 on CHO cells resulted in up to 80% rosette formation, indicating synergistic adhesion.
- CD59 enhanced T cell proliferation sevenfold, CD58 enhanced it 20-fold, and combined expression led to over 40-fold enhancement.
Conclusions:
- Both CD58 and CD59 molecules directly contribute to T cell adhesion and activation.
- These molecules exhibit synergistic function when co-expressed on accessory cells, significantly amplifying T cell responses.