Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse fluorescent chromosome banding with chromomycin and DAPI.

D Schweizer

    Chromosoma
    |November 29, 1976
    PubMed
    Summary

    Chromomycin A3 (CMA) and mithramycin (MM) act as guanine-specific fluorescent chromosome dyes. These dyes reveal distinct heterochromatin patterns in plants and humans, offering insights into chromosome structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Annular distribution patterns of .357 Magnum fragments in soft tissue simulants after striking hard material that prevented the bullet from exiting.

    Journal of forensic and legal medicine·2021
    Same author

    Nuclear DNA contents of Pisum genotypes grown in vivo and in vitro.

    Planta·2014
    Same author

    Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement.

    Plant cell reports·2013
    Same author

    Improved contrast deep optoacoustic imaging using displacement-compensated averaging: breast tumour phantom studies.

    Physics in medicine and biology·2011
    Same author

    Integration of common bean ( Phaseolus vulgaris L.) linkage and chromosomal maps.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2003
    Same author

    Exciting future planned for birthplace of genetics.

    Nature·2001

    Area of Science:

    • Molecular Biology
    • Cytogenetics
    • Biochemistry

    Background:

    • Chromomycin A3 (CMA) and mithramycin (MM) are guanine-specific DNA-binding antibiotics.
    • Fluorescent dyes are crucial for visualizing chromosome structure and organization.

    Purpose of the Study:

    • To investigate the utility of CMA and MM as fluorescent chromosome stains.
    • To compare CMA/MM staining patterns with DAPI (4',6-diamidino-2-phenylindole) staining.

    Main Methods:

    • Sequential staining of plant (Vicia faba, Scilla siberica, Ornithogalum caudatum) and human metaphase chromosomes.
    • Use of CMA or MM in conjunction with DAPI, and counterstains like methyl green or actinomycin D (AMD).

    Main Results:

    • CMA and MM intensely fluoresced nucleolus organizer regions and associated heterochromatin in plants.
    • Heterochromatic segments bright with CMA/MM were typically pale with DAPI, and vice versa.
    • Human chromosomes showed CMA fluorescence patterns that were largely the reverse of AMD/DAPI banding.

    Conclusions:

    • CMA and MM are effective fluorescent chromosome dyes, highlighting GC-rich regions.
    • The CMA banding pattern in humans resembles R-banding, suggesting its potential in cytogenetic analysis.

    Related Experiment Videos