Caffeine potentiation on lethality of L-1210 cells treated with neocarzinostatin

The caffeine potentiation on lethality of L-1210 cells treated with Neocarzinostatin was studied. Colony formation of L-1210 cells treated with Neocarzinostatin was significantly decreased by incubation of the cells for 48 hr with 0.5 mM of caffeine which alone did not affect the growth rate and colony-forming ability of the cells. These results indicate that Neocarzinostatin may induce repairable damage in DNA of L-1210 cells.