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Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve
K V Baev1, V K Beresovskii, V N Kalunov
1Department of Physiology of the Spinal Cord, A. A. Bogomoletz Institute of Physiology, Academy of Sciences, Ukrainian SSR, Kiev.
Abstract:
Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed. Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype.