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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Published on: May 4, 2012
Human centrosomal epitope is shared specifically with human lactate dehydrogenase-B isozyme
1Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.
FEBS Letters
|March 16, 1992
Summary
Rabbit serum 0013 identifies a specific epitope on human lactate dehydrogenase B (LDH-B) subunits. This finding provides the most potent anti-LDH-B serum available for research.
Area of Science:
- Cell Biology
- Biochemistry
- Immunology
Background:
- Rabbit serum 0013 was initially used to identify centrosomal proteins.
- The serum also recognized a 36 kDa non-centrosomal protein, identified as human lactate dehydrogenase (LDH).
- The co-distribution of the 0013 epitope on centrosomal proteins and LDH was found to be specific to human cells, with human hepatic cells being an exception.
Purpose of the Study:
- To investigate the specificity of the 0013 epitope in human cells.
- To determine the precise target of the 0013 epitope within lactate dehydrogenase.
- To characterize the anti-LDH-B activity of serum 0013.
Main Methods:
- Immunological assays using rabbit serum 0013.
- Analysis of human hepatic cells expressing only the LDH-A4 isozyme.
- Characterization of lactate dehydrogenase subunits.
Main Results:
- The 0013 epitope is specific for the human LDH-B subunit.
- Serum 0013 demonstrates high specificity for LDH-B.
- Human hepatic cells, expressing only LDH-A4, confirmed the epitope's specificity for LDH-B.
Conclusions:
- Serum 0013 is a highly specific reagent for detecting the human LDH-B subunit.
- This specificity offers a valuable tool for studying LDH-B in various biological contexts.
- The findings prompt further investigation into the evolutionary and physiological significance of LDH-B specificity.
