Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Okadaic acid is a potent angiogenesis inducer.

T Oikawa1, M Suganuma, H Ashino-Fuse

  • 1Division of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science.

Japanese Journal of Cancer Research : Gann
|January 1, 1992
PubMed
Summary

Okadaic acid, a protein phosphatase inhibitor, significantly promotes angiogenesis in chick embryos. This suggests a novel pathway for inducing blood vessel formation, distinct from TPA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Fat Diet-Induced Diabetic Conditions Exacerbate Cognitive Impairment in a Mouse Model of Alzheimer's Disease Via a Specific Tau Phosphorylation Pattern.

The journal of prevention of Alzheimer's disease·2024
Same author

Dyschromatosis symmetrica hereditaria with chilblains due to a novel two-amino-acid deletion in the double-stranded RNA-binding domain of ADAR1.

Journal of the European Academy of Dermatology and Venereology : JEADV·2018
Same author

Bilateral striatal necrosis and dyschromatosis symmetrica hereditaria: A-I editing efficiency of ADAR1 mutants and phenotype expression.

The British journal of dermatology·2018
Same author

Dowling-Degos disease with mutations in POFUT1 is clinicopathologically distinct from reticulate acropigmentation of Kitamura.

The British journal of dermatology·2015
Same author

Abrogation of protein phosphatase 6 promotes skin carcinogenesis induced by DMBA.

Oncogene·2014
Same author

Studies on expression of aldehyde dehydrogenase in normal and cancerous tissues of thyroids.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2014

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • Okadaic acid is a tumor promoter and inhibitor of protein phosphatases 1 and 2A.
  • Tumor promoters can induce angiogenesis, the formation of new blood vessels.
  • Understanding the mechanisms of angiogenesis is crucial for cancer research.

Purpose of the Study:

  • To investigate the angiogenic potential of okadaic acid.
  • To compare the angiogenic activity of okadaic acid with that of TPA (12-O-tetradecanoylphorbol-13-acetate).
  • To elucidate the mechanism of action for okadaic acid-induced angiogenesis.

Main Methods:

  • Chick embryo chorioallantoic membrane assay was used to assess angiogenesis.
  • Dose-response studies were conducted to determine effective doses.

Related Experiment Videos

  • Time-course analysis was performed to compare induction rates with TPA.

    • Main Results:

    • Okadaic acid induced dose-dependent angiogenesis in the chick embryo chorioallantoic membrane.
    • The minimum effective dose was 5 fmol/egg, and the ED50 was 90 fmol/egg.
    • Okadaic acid's angiogenic activity was one order of magnitude stronger than TPA's, but its induction time-course was slower.

    Conclusions:

    • Okadaic acid exhibits potent angiogenic activity, exceeding that of TPA.
    • The slower time-course of angiogenesis induction by okadaic acid suggests a different mechanism of action compared to TPA.
    • These findings indicate a novel pathway for angiogenesis induction by okadaic acid, distinct from the TPA pathway.