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Isolation of viruses from children with infectious hepatitis

E V DAVIS

    Science (New York, N.Y.)
    |June 30, 1961
    PubMed

    Insights

    Researchers isolated unknown cytopathologic agents from Indian children during an infectious hepatitis outbreak in Arizona. These agents, cultured in human embryonic lung cells, could not be identified as known viruses using standard methods.

    Area of Science:

    • Hepatology
    • Virology
    • Cell Biology

    Background:

    • An outbreak of infectious hepatitis occurred among Indian children in Arizona.
    • Cytopathologic agents were suspected to be the cause of the outbreak.

    Purpose of the Study:

    • To isolate and identify the causative agents of infectious hepatitis in the affected children.
    • To determine if the isolated agents were known viruses.

    Main Methods:

    • Isolation of cytopathologic agents from clinical samples of affected children.
    • Cultivation of agents in a serially transplantable human embryonic lung cell line.
    • Attempts to identify the agents using standard virologic techniques.

    Main Results:

    • Cytopathologic agents were successfully isolated from 14 out of 22 children.
    • The agents could be propagated in the human embryonic lung cell line.
    • Standard identification methods failed to identify the agents as known viruses.

    Conclusions:

    • An unknown cytopathologic agent was implicated in the infectious hepatitis outbreak.
    • Further research is needed to characterize and identify this novel agent.
    Keywords:
    HEPATITIS, INFECTIOUS/virology

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