Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A sensitive immunoassay for porcine interferon-alpha.

H Diaz de Arce1, K Artursson, R L'Haridon

  • 1Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.

Veterinary Immunology and Immunopathology
|January 31, 1992
PubMed
Summary

A new ELISA assay effectively measures porcine interferon-alpha (IFN-alpha) in cell cultures and serum. This assay offers improved precision over traditional bioassays for quantifying this important immune protein.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Longitudinal study of Staphylococcus aureus genotypes isolated from bovine clinical mastitis" (J. Dairy Sci. 104: 11945-11954).

Journal of dairy science·2025
Same author

[The bovine spongiform encephalopathy in the UK and France: an epidemiological perspective].

Virologie (Montrouge, France)·2021
Same author

Longitudinal study of Staphylococcus aureus genotypes isolated from bovine clinical mastitis.

Journal of dairy science·2021
Same author

Biological and antigenic relationships between virus-induced porcine and human interferons.

Annales de l'Institut Pasteur. Virology·2020
Same author

Inhibition of transmissible gastroenteritis coronavirus (TGEV) multiplication <i>in vitro</i> by non-immune lymphocytes.

Annales de l'Institut Pasteur. Virology·2020
Same author

Enquête sérologique par immunofluorescence indirecte, sur les infections à coronavirus OC<sub>43</sub>.

Annales de l'Institut Pasteur. Virology·2020

Area of Science:

  • Immunology
  • Biotechnology
  • Veterinary Science

Background:

  • Porcine interferon-alpha (IFN-alpha) plays a crucial role in antiviral immunity.
  • Accurate quantification of IFN-alpha is essential for research and diagnostics.
  • Existing bioassays for IFN-alpha can lack precision and throughput.

Purpose of the Study:

  • To develop and validate a sensitive and precise enzyme-linked immunosorbent assay (ELISA) for quantifying porcine IFN-alpha.
  • To compare the performance of the developed ELISA with a standard bioassay.

Main Methods:

  • Development of a two-site ELISA using murine monoclonal antibodies against distinct porcine IFN-alpha epitopes.
  • Optimization of the ELISA for both cell culture supernatant and porcine serum samples.

Related Experiment Videos

  • Comparison of ELISA results with a cytopathic effect inhibition bioassay.

    • Main Results:

    • The one-step ELISA detected 0.5 units/mL of IFN-alpha in cell culture medium with high precision.
    • A two-step ELISA version achieved a sensitivity of 1 unit/mL for porcine serum, despite some serum factors reducing sensitivity.
    • ELISA results generally correlated well with the bioassay, with minor discrepancies in a small number of samples.

    Conclusions:

    • The developed two-site ELISA is a sensitive and precise method for quantifying porcine IFN-alpha in various biological matrices.
    • The ELISA offers advantages in precision and potentially throughput compared to traditional bioassays.
    • Further validation is recommended to address minor discrepancies observed between ELISA and bioassay results.