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Expression of CD54, CD58, CD14, and HLA-DR on macrophages and macrophage-derived accessory cells and their accessory
G Ocklind1, D Friedrichs, J H Peters
1Department of Zoophysiology, Uppsala University, Sweden.
Abstract:
Human peripheral monocytes can differentiate in vitro into macrophages (Mph) possessing a low accessory activity in T cell stimulation. Mph can be converted into a state of high accessory activity by treatment with dibutyryl cyclic AMP. This finding was used in this study to achieve Mph-derived AC (MphAC). Among the surface antigens on AC which have been shown to participate in accessory events leading to T cell proliferation, MHC class II antigens, CD58 (LFA-3) and CD54 (ICAM-1) seem to be especially important. We show here that the high accessory capacity of MphAC was not correlated with a high level of the surface antigens HLA-DR, CD58, and CD54. The amount of CD54 molecules was, in fact, lower on the MphAC than on the Mph.
Insights
Human monocytes differentiate into macrophages with low T cell accessory activity. Treatment with dibutyryl cyclic AMP converts these macrophages into Mph-derived AC with high accessory capacity, independent of certain surface antigen levels.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human peripheral monocytes differentiate into macrophages (Mph) with limited accessory activity in T cell stimulation.
- Macrophage accessory function can be enhanced by specific treatments.
Purpose of the Study:
- To investigate the accessory capacity of Mph-derived AC (MphAC) generated by dibutyryl cyclic AMP treatment.
- To correlate the accessory function of MphAC with the expression of key surface antigens involved in T cell proliferation.
Main Methods:
- Differentiating human peripheral monocytes into macrophages in vitro.
- Treating macrophages with dibutyryl cyclic AMP to generate Mph-derived AC.
- Analyzing the expression levels of surface antigens (HLA-DR, CD58, CD54) on Mph and MphAC.
- Assessing the accessory activity of MphAC in T cell stimulation.
Main Results:
- Mph-derived AC (MphAC) exhibit high accessory capacity for T cell stimulation.
- The enhanced accessory capacity of MphAC is not directly correlated with high expression of HLA-DR, CD58, or CD54.
- Interestingly, MphAC displayed lower levels of CD54 (ICAM-1) compared to the original Mph.
Conclusions:
- Dibutyryl cyclic AMP effectively enhances the accessory function of Mph-derived AC.
- Key surface antigens like HLA-DR, CD58, and CD54 are not the sole determinants of the enhanced accessory capacity in MphAC.
- Further investigation is needed to understand the mechanisms underlying the high accessory function of MphAC, especially concerning CD54 expression.