Related Experiment Videos
Amplification of viral RNA for the detection of dengue types 1 and 2 virus
1Department of Biochemistry, Chang Gung Medical College, Taipei, Taiwan, Republic of China.
The Journal of Infection
|January 1, 1992
Abstract:
In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types 1 and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.