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Related Experiment Videos

Endothelial function in thrombosis and thrombolysis.

K Sueishi1, C Yasunaga, T Murata

  • 1Department of Pathology, Faculty of Medicine, Kyusyu University, Fukuoka, Japan.

Japanese Circulation Journal
|February 1, 1992
PubMed
Summary

Tissue factor (TF) is found in atherosclerotic plaques, primarily in macrophages. Plasminogen activators, especially urokinase-type PA (uPA), enhance blood vessel formation (angiogenesis) by promoting endothelial cell migration.

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Area of Science:

  • Cardiovascular Biology
  • Cellular Biology
  • Hemostasis and Thrombosis

Background:

  • Tissue Factor (TF) initiates the extrinsic coagulation pathway and is implicated in atherosclerosis.
  • Macrophages are increasingly recognized as key players in atherosclerotic plaque development.
  • Angiogenesis, the formation of new blood vessels, is a critical process in wound healing and disease.

Purpose of the Study:

  • To investigate the localization of TF in human aortic atherosclerotic plaques.
  • To explore the role of plasminogen and plasminogen activators (PAs) in angiogenesis in vitro.
  • To examine the relationship between fibrinolysis and angiogenesis.

Main Methods:

  • Immunohistochemistry was used to detect TF in human aortic atherosclerotic plaques.

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  • A quantitative in vitro assay using endothelial cell cultures on collagen gel was developed to study angiogenesis.
  • Morphometric analysis quantified endothelial cell migration and angiogenic activity.

    • Main Results:

    • TF was ubiquitously present in atherosclerotic intima, predominantly expressed by macrophages, not endothelial cells.
    • Endothelial cells cultured on collagen gel demonstrated enhanced angiogenic activity in the presence of plasminogen and PAs, particularly urokinase-type PA (uPA).
    • uPA was observed to be cell-associated, likely via its receptors on endothelial cells, suggesting a role in migration.

    Conclusions:

    • Macrophages in atherosclerotic plaques are a significant source of TF.
    • Endothelial cell-derived uPA plays a crucial role in promoting angiogenesis by enhancing endothelial cell migration.
    • The regulation of pericellular fibrinolysis is closely linked to the process of angiogenesis.