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Induced RAW 264.7 macrophages express soluble and particulate nitric oxide synthase: inhibition by transforming
U Förstermann1, H H Schmidt, K L Kohlhaas
1Abbott Laboratories, Abbott Park, IL 60064.
European Journal of Pharmacology
|February 13, 1992
Abstract:
RAW 264.7 macrophages induced with lipopolysaccharide and interferon-gamma expressed nitric oxide (NO) synthase. Approximately two-thirds of the total induced NO synthase activity was found in the cytosolic fraction, whereas one-third was associated with the particulate fraction. Both enzymes formed L-citrulline in addition to NO-like material. NO and L-citrulline formation by both enzymes were calcium-independent and inhibited by NG-nitro-L-arginine and NG-methyl-L-arginine. Transforming growth factor-beta 1 prevented the induction of both enzymes.