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The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides with

E Yamamura1, H Hirono, M Takeuchi

  • 1Toxicology Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Fukuoka-ken, Japan.

Mutation Research
|February 1, 1992
PubMed

Insights

A novel micronucleus assay using mouse peripheral blood and acridine orange (AO) staining was validated. This method offers a reliable alternative to traditional bone marrow assays for genotoxicity testing.

Area of Science:

  • Toxicology
  • Genetics
  • Hematology

Background:

  • Micronucleus assays are crucial for detecting genotoxicity.
  • Conventional methods often utilize bone marrow, which can be invasive.
  • Developing alternative, less invasive methods is important for toxicological studies.

Purpose of the Study:

  • To validate a novel micronucleus assay using mouse peripheral blood.
  • To assess the efficacy of supravital staining with acridine orange (AO) for this assay.
  • To establish this method as a viable alternative to the conventional bone marrow assay.

Main Methods:

  • Validation of a micronucleus assay in peripheral blood from triethylenemelamine-treated mice.
  • Utilized supravital staining with acridine orange (AO).
  • Assay performed and validated across two independent laboratories.

Main Results:

  • Observed dose-dependent increases in micronucleated peripheral reticulocytes.
  • Demonstrated time-dependent increases in micronucleated peripheral reticulocytes.
  • Consistent results were obtained from both validating laboratories.

Conclusions:

  • The validated peripheral blood micronucleus assay using AO is effective.
  • This method provides a reliable and potentially less invasive alternative to bone marrow assays.
  • The assay is suitable for genotoxicity assessment in toxicological research.

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