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A new quantitative polymerase chain reaction-high performance ion exchange liquid chromatographic method for the
J F Schmitt1, M Guthridge, C Economou
1Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Abstract:
A method for the quantitative determination of fibroblast growth factor-beta (FGF-beta) genomic amplification based on the use of optimized polymerase chain reaction (PCR) procedures and high performance ion exchange (HPIEX) liquid chromatography has been developed. Co-amplification of a second genomic species permits internal standardization of the techniques during optimization of the reaction conditions, the PCR cycle number and the PCR cycle efficiency, as well as during the analytical HPIEX chromatographic determination of the PCR products. These investigations confirm the versatility of these procedures to quantitatively analyse FGF-beta gene amplification in various cells and tissues.