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Mouse MRP8 and MRP14, two intracellular calcium-binding proteins associated with the development of the myeloid

E Lagasse1, I L Weissman

  • 1Department of Pathology, Howard Hughes Medical Institute, Stanford University School of Medicine, CA 94305.

Blood
|April 15, 1992
PubMed

Insights

Mouse myeloid progenitor cells express MRP8 and MRP14 proteins, crucial for inflammatory disorders. These calcium-binding proteins are found in immature myeloid cells but are lost during terminal differentiation into macrophages.

Area of Science:

  • Immunology
  • Molecular Biology
  • Hematopoiesis

Background:

  • MRP8 and MRP14 are S100-like calcium-binding proteins implicated in human inflammatory disorders.
  • Their precise function and expression patterns, particularly during hematopoiesis, remain largely unknown.

Purpose of the Study:

  • To clone mouse MRP8 and MRP14 and characterize their expression patterns during hematopoiesis.
  • To investigate the role of MRP8 and MRP14 in myeloid cell development and inflammatory responses.

Main Methods:

  • Cloning of mouse MRP8 and MRP14 genes.
  • Analysis of protein expression via immunohistochemistry and flow cytometry.
  • Investigation of expression in fetal and adult hematopoietic tissues, including bone marrow, fetal liver, and spleen.
  • Examination of expression in thioglycollate-induced peritoneal inflammatory exudates.

Main Results:

  • Mouse MRP8 and MRP14 share 59% identity with human counterparts and are coexpressed in fetal myeloid progenitors by day 11 of gestation.
  • MRP+ cell populations increase with myeloid lineage development in fetal liver and yolk sac.
  • In adult mice, MRP8 and MRP14 are found in immature myeloid cells, monocytes, and neutrophils, but not in terminally differentiated macrophages.
  • Expression is significantly upregulated in neutrophils and monocytes recruited to inflammatory sites.

Conclusions:

  • MRP8 and MRP14 are key molecular markers of immature myeloid cells and inflammatory infiltrates in mice.
  • Their expression dynamics suggest a role in myeloid cell development and inflammatory processes.
  • Understanding MRP8/MRP14 expression provides insights into the cellular mechanisms underlying inflammatory disorders.

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