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A coagglutination assay with monoclonal antibodies for rapid laboratory identification of Mycoplasma synoviae
M A Morsy1, V S Panangala, M M Gresham
1Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama.
Avian Diseases
|January 1, 1992
Abstract:
An agglutinating monoclonal antibody (MAb S2) specific for a 55,000-molecular-weight surface protein of Mycoplasma synoviae was developed by fusion of spleen cells from immunized BALB/c mice with P3X63 Ag8.653 myeloma cells. Immunogold labeling experiments confirmed the cell surface location of the MAb-recognized antigen. MAb S2-coated Staphylococcus aureus was used in a rapid slide coagglutination assay. Eleven M. synoviae strains, including the type strain WVU 1853, coagglutinated with MAb-coated S. aureus, but five M. gallisepticum strains (PG31, S6, R, F, and A5969) did not.