Related Experiment Videos
Electrophoresis and silver staining of rat urinary proteins including alpha 2u-globulin (rat n II)
T Marshall1, K M Williams, C Bayard
1Biochemistry Research Laboratory, School of Pharmaceutical and Chemical Sciences, Sunderland Polytecnic, UK.
Applied and Theoretical Electrophoresis : the Official Journal of the International Electrophoresis Society
|January 1, 1992
Abstract:
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining have been used to monitor the fractionation of rat urinary proteins by Amicon Centriprep C30/C10 ultrafiltration and to assess the purification of alpha 2u-globulin, a laboratory animal allergen, precipitated by exhaustive dialysis. Factors influencing the protein composition of rat urine have been identified. Dialysis procedures have been evaluated for precipitation of alpha 2u-globulin and ultrafiltration used for further purification of the allergen.