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A recA-ada hybrid gene inducible by DNA damage
K Kleibl1, G P Margison, M Skorvaga
1Department of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Mutation Research
|May 1, 1992
Abstract:
A damage-inducible expression vector was constructed in which the original recA structural gene was replaced by the protein-coding region of the ada gene. The O6-alkylguanine-DNA alkyltransferase encoded by the ada gene can be measured by a rapid and highly sensitive assay. The introduction of this construct into an appropriate host cell provides an effective bacterial assay for genotoxins.