Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader.

F Galgani1, Y Cadiou, F Gilbert

  • 1IFREMER, Nantes, France.

Ecotoxicology and Environmental Safety
|April 1, 1992
PubMed
Summary

This study introduces an iterative method for determining toxicant concentrations (IC50) in marine algae like Tetraselmis suesica using plate reader data. The novel approach accurately estimates toxicity, aiding environmental risk assessment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond Threat: Changes in Visuocortical Engagement and Oscillatory Brain Activity during Non-Aversive Associative Learning.

bioRxiv : the preprint server for biology·2025
Same author

Door-to-puncture time in ischemic stroke with large vessel occlusion in France: Patient and hospital factors.

Revue neurologique·2025
Same author

Monitoring macroplastics in aquatic and terrestrial ecosystems: Expert survey reveals visual and drone-based census as most effective techniques.

The Science of the total environment·2024
Same author

Bioindicator species of plastic toxicity in tropical environments.

The Science of the total environment·2024
Same author

Assessing marine litter in a highly polluted area in the Mediterranean: A multi-perspective approach in the Saronikos Gulf, Greece.

Marine pollution bulletin·2024
Same author

Paraffin waxes in the North-Western Mediterranean Sea: A comprehensive assessment in the Pelagos Sanctuary, a Specially Protected Area of Mediterranean Importance.

Journal of hazardous materials·2024

Area of Science:

  • Environmental Toxicology
  • Marine Biology
  • Biochemical Assays

Background:

  • Accurate toxicity testing is crucial for environmental risk assessment.
  • Marine unicellular algae are sensitive indicators of aquatic pollution.
  • Standard toxicity assays often require optimization for efficiency and precision.

Purpose of the Study:

  • To develop and validate an iterative system for determining median lethal concentration (LC50) or half maximal inhibitory concentration (IC50).
  • To utilize a marine unicellular alga, Tetraselmis suesica, as a model organism for toxicity assessment.
  • To establish a robust method for analyzing dose-response relationships in ecotoxicology.

Main Methods:

  • Employing a plate reader to measure esterase activity of Tetraselmis suesica using fluorescein diacetate.

Related Experiment Videos

  • Utilizing an iterative process with a sigmoid function to analyze dose-response data.
  • Estimating IC50 values with associated standard error of the mean (SEM) and statistical significance (P < 0.05).

    • Main Results:

    • The iterative system successfully determined IC50 values for toxicants.
    • The method demonstrated reliable estimation of dose-response relationships.
    • Phosalone was used as a representative toxicant to validate the application.

    Conclusions:

    • The described iterative system provides an efficient and accurate method for LC50/IC50 determination in marine algae.
    • This approach enhances the reliability of ecotoxicological assessments.
    • The system is applicable for evaluating the toxicity of various chemical agents in aquatic environments.