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Related Experiment Videos

CD5-negative cell mediated experimental hypersensitivity pneumonitis.

M R Schuyler1, K Gott, G Shopp

  • 1Department of Medicine, Albuquerque Veterans Administration Medical Center, University of New Mexico School of Medicine.

The American Review of Respiratory Disease
|May 1, 1992
PubMed
Summary
This summary is machine-generated.

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Peripheral lymph node cells cultured with antigen can transfer experimental hypersensitivity pneumonitis (EHP). Depleting CD5+ cells from these cultured cells reduced their ability to transfer EHP, suggesting a role for T cells.

Area of Science:

  • Immunology
  • Pulmonary Medicine

Background:

  • Experimental hypersensitivity pneumonitis (EHP) is transferable via peripheral lymph node (PLN) cells in Strain 2 guinea pigs.
  • The specific cell types responsible for EHP transfer remain unclear.
  • The potential of lung-associated lymph node (LALN) cells to transfer EHP after in vitro culture is unknown.

Purpose of the Study:

  • To delineate the phenotype of cells mediating EHP transfer.
  • To investigate if cultured LALN cells can transfer EHP.
  • To determine the role of CD5+ cells in EHP transfer.

Main Methods:

  • PLN and LALN cells were cultured with Micropolyspora faeni antigen.
  • Blast cells were isolated, and PLN cells were fractionated using nylon wool columns.
  • CD5+ cells were depleted from PLN blast cell populations using anti-CD5 antibody and complement.

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Main Results:

  • Cultured PLN cells transferred EHP to recipient guinea pigs.
  • The nonadherent PLN cell fraction was enriched for CD5+ (T) cells.
  • Depletion of CD5+ cells significantly reduced the pulmonary response to M. faeni challenge.

Conclusions:

  • Cultured PLN cells can transfer EHP.
  • CD5+ T cells are crucial for the transfer of EHP.
  • Further research into LALN cell function in EHP is warranted.