Ex vivo expansion of peripheral blood progenitor cells with recombinant cytokines

Peripheral blood mononuclear cells were isolated and cultured in the presence of Steel factor and/or PIXY321, a GM-CSF/IL-3 fusion protein. We compared the number of colony forming cells (CFC) per culture on day zero to the number of CFC after liquid culture in the presence of these cytokines. After a four day incubation with PIXY321 and Steel factor the number of CFU-GM increased 5.6-fold and the number of BFU-E increased 2.2-fold in four separate experiments. The expansion on day 8 post incubation was 16.1-fold for myeloid colony formation and 9.7-fold for erythroid colony formation. These studies demonstrate the potential to expand CFC from peripheral blood with a simple ex vivo culture procedure.