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Corrected nucleotide sequence of M13mp18 gene III.
1Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855-0759.
Gene
|May 1, 1992
Summary
Seven nucleotide sequence differences were found in bacteriophage M13mp18 gene III compared to its previously reported sequence. This finding clarifies the precise genetic makeup of this important bacteriophage research tool.
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Bacteriophage M13 is a widely used filamentous phage in molecular biology.
- Gene III encodes a key structural protein involved in phage infection.
- Accurate nucleotide sequences are crucial for genetic manipulation and research.
Purpose of the Study:
- To identify and report discrepancies in the nucleotide sequence of bacteriophage M13mp18 gene III.
- To provide an accurate reference sequence for bacteriophage M13mp18 gene III.
Main Methods:
- Comparative analysis of nucleotide sequences.
- Bioinformatic tools for sequence alignment and identification of differences.
Main Results:
- Seven specific nucleotide differences were identified between the actual and previously reported sequence of bacteriophage M13mp18 gene III.
- The previously reported sequence was based on wild-type bacteriophage M13 gene III, leading to the observed variations.
Conclusions:
- The study highlights the importance of sequence verification in molecular biology research.
- The corrected sequence information for bacteriophage M13mp18 gene III is vital for researchers utilizing this vector.