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Analysis of tick-borne encephalitis virus antigens by monoclonal antibodies
L Grubhoffer1, J Kopecký, E Tomková
1Institute of Parasitology, Czechoslovak Academy of Sciences, Budĕjovice.
Microbios
|January 1, 1992
Abstract:
Monoclonal antibodies (MAbs) against Central European tick-borne encephalitic virus, strain Hypr, were used for determination and characterization of viral antigens of infected PS cells. The MAbs reacted in immunoblotting with flavivirus glycoprotein E (56 kD), and nonstructural protein NS3 (70 kD). According to enzyme immunoassay with infected cells, NS3 antigen is expressed in the plasmalemma.