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Related Experiment Videos

Chorionic villus sampling for fetal Rh typing: clinical implications.

T S Kickler1, K Blakemore, R S Shirey

  • 1Department of Laboratory Medicine, Johns Hopkins University School of Medicine, Baltimore, MD.

American Journal of Obstetrics and Gynecology
|May 1, 1992
PubMed
Summary

Early prenatal Rh typing using chorionic villus sampling is possible as early as 9 to 11 weeks' gestation. This immune rosette technique accurately determines fetal Rh status, aiding in managing Rh incompatibility during pregnancy.

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Area of Science:

  • Maternal-fetal medicine
  • Genetics
  • Immunology

Background:

  • Rh incompatibility is a significant concern in pregnancy.
  • Accurate and early fetal Rh typing is crucial for management.
  • Chorionic villus sampling (CVS) offers early fetal tissue for analysis.

Purpose of the Study:

  • To evaluate the feasibility of prenatal Rh phenotyping using red blood cells from CVS.
  • To determine the earliest gestational age for reliable Rh typing via CVS.
  • To assess the utility of this technique in managing Rh-negative pregnancies.

Main Methods:

  • Immune rosette technique was employed for Rh typing.
  • Red blood cells were obtained from chorionic villi via CVS (9-11 weeks gestation).
  • Fetal origin of samples was confirmed using radial immunodiffusion for fetal hemoglobin.

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Main Results:

  • Accurate Rh phenotyping was achieved with 2-8 mg of chorionic villi.
  • Samples were confirmed to be >90% fetal in origin.
  • Antenatal fetal Rh typing correlated with postnatal confirmation in 9 out of 10 pregnancies carried to term.

Conclusions:

  • Prenatal Rh phenotyping is reliable as early as 9-11 weeks gestation using CVS.
  • This early typing method can significantly aid in the management of Rh-incompatible pregnancies.
  • The immune rosette technique provides a valuable tool for early fetal Rh status determination.