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Multilocular renal cyst. Immunohistochemical and lectin-binding study.
R M Davila1, J M Kissane, E C Crouch
1Department of Pathology, Jewish Hospital of St. Louis, MO 63110.
The American Journal of Surgical Pathology
|May 1, 1992
Summary
Multilocular renal cysts (MRC) exhibit aberrant glycoprotein expression in lining cells, resembling distal tubules but with some proximal markers. These uncommon kidney lesions show low cell proliferation and activated stromal cells.
Area of Science:
- Nephrology
- Pathology
- Biochemistry
Background:
- Multilocular renal cyst (MRC) is a rare kidney lesion with unclear origins.
- Previous studies have not fully elucidated the cellular and molecular characteristics of MRC.
Purpose of the Study:
- To investigate the immunohistochemical and lectin-binding profiles of multilocular renal cysts in adult patients.
- To understand the pathogenesis of MRC by examining epithelial and stromal cell markers.
Main Methods:
- Immunohistochemistry was used to analyze keratin, epithelial membrane antigen, Ber-EP4, alpha-1-antitrypsin, lysozyme, and proliferating cell nuclear antigen.
- Lectin binding assays with Arachis hypogaea and Lotus tetragonolobus were performed.
- Staining for type IV collagen and type I procollagen assessed basement membrane and stromal activity.
Main Results:
- Lining epithelial cells showed strong keratin and Arachis hypogaea lectin binding, similar to distal tubules.
- Variable expression of distal nephron markers (EMA, Ber-EP4) and coexpression of proximal markers (alpha-1-antitrypsin, lysozyme, Lotus tetragonolobus lectin) were observed.
- Continuous basement membrane and activated stromal cells with procollagen deposition were present; low epithelial and stromal proliferation was detected.
Conclusions:
- Multilocular renal cysts display aberrant tubular epithelial glycoprotein and glycoconjugate expression.
- These lesions are characterized by low proliferative activity and associated activation of interlocular stromal cells.
- The findings suggest a complex origin possibly involving differentiation abnormalities of renal tubular cells.