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CD20 and CD5 expression in B-chronic lymphocytic leukemia.
G E Marti1, G Faguet, P Bertin
1Laboratory of Cellular and Molecular Biology, Food and Drug Administration, National Institutes of Health, Bethesda, Maryland 20892.
Annals of the New York Academy of Sciences
|May 4, 1992
Summary
Researchers quantified decreased CD20 fluorescence intensity on B-cell chronic lymphocytic leukemia (B-CLL) lymphocytes. This reduction in CD20 expression on B-CLL cells may be linked to CD5 expression, suggesting genetic involvement.
Area of Science:
- Immunology
- Hematology
- Molecular Biology
Background:
- Previous observations noted a decrease in CD20 fluorescence intensity (FI) on B-cell chronic lymphocytic leukemia (B-CLL) lymphocytes.
- Quantifying this decrease is crucial for understanding B-CLL cell surface marker expression.
Purpose of the Study:
- To quantitatively assess the reduced CD20 fluorescence intensity on B-CLL lymphocytes.
- To investigate the relationship between CD5 and CD20 expression in B-CLL.
Main Methods:
- Calculation of binding capacities (BC x 10^3) to quantify CD20 antibody binding on normal peripheral blood lymphocytes (B-PBL) and B-CLL lymphocytes.
- Qualitative assessment of CD5 expression density on B-PBL and B-CLL compared to normal T cells.
- Analysis of CD5 and CD20 coexpression patterns.
Main Results:
- Binding capacities for CD20 reagents confirmed decreased CD20 expression on B-CLL lymphocytes compared to normal B-PBL.
- CD5 expression on B-CLL lymphocytes was qualitatively lower than on normal T cells.
- CD5 expression on B-CLL appears linked to lower CD20 levels, with CD5 potentially absent on B-CLL expressing normal CD20 levels.
Conclusions:
- The decreased levels of CD20 in B-CLL suggest the involvement of one or two genes (alleles).
- This decreased CD20 expression may be genetically linked to the observed CD5 expression patterns in B-CLL.