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Related Experiment Videos

SAG: a Schwann cell membrane glycoprotein.

M E Dieperink1, A O'Neill, G Magnoni

  • 1Department of Neurology, University of Chicago, Illinois 60637.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|June 1, 1992
PubMed
Summary

A novel Schwann cell membrane glycoprotein (SAG), containing HNK-1 carbohydrate, is exclusively found in the peripheral nervous system (PNS). SAG expression in tumors depends on the presence of axons, suggesting its role in Schwann cell-axon interactions.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • The peripheral nervous system (PNS) relies on Schwann cells for nerve function and myelination.
  • Specific cell surface molecules mediate critical Schwann cell interactions with axons.
  • The HNK-1 carbohydrate epitope is found on various adhesion molecules, but its precise role in the PNS is not fully understood.

Purpose of the Study:

  • To characterize a novel glycoprotein, designated Schwann cell membrane glycoprotein (SAG), identified exclusively in the PNS.
  • To investigate the expression patterns of SAG in developing nerves and Schwann cell tumors.
  • To explore the regulation of SAG expression in relation to axonal presence and cellular environment.

Main Methods:

  • Biochemical characterization of a 170,000 Da glycoprotein.

Related Experiment Videos

  • Immunohistochemical analysis of rat sciatic nerve during development.
  • Analysis of SAG expression in neurofibromas and schwannomas.
  • In vitro studies using schwannoma cells cultured with and without PC12 neuronal cells, and with forskolin treatment.

    • Main Results:

    • A 170,000 Da glycoprotein, SAG, was identified and found exclusively in the PNS.
    • SAG contains the HNK-1 carbohydrate and has a unique N-terminal sequence.
    • SAG is present in rat sciatic nerve before myelination and in both myelinating and nonmyelinating Schwann cells.
    • SAG is expressed in neurofibromas (Schwann cell tumors with axons) but not in schwannomas (tumors lacking axons).
    • Schwannoma cells in culture do not express SAG, even when stimulated with forskolin, but do express SAG when co-cultured with PC12 neuronal cells.

    Conclusions:

    • SAG is a novel PNS-specific glycoprotein potentially involved in Schwann cell-axon interactions.
    • The expression of SAG is regulated by the presence of axons, highlighting its role in nerve development and maintenance.
    • SAG represents a new marker for Schwann cell-related tissues and may play a role in cell adhesion or signaling.