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Nucleotide sequence of rat vascular cell adhesion molecule-1 cDNA
A J Williams1, R C Atkins, J W Fries
1Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Biochimica Et Biophysica Acta
|June 25, 1992
Summary
Researchers cloned and sequenced rat Vascular Cell Adhesion Molecule 1 (VCAM-1). This protein is crucial for leukocyte and tumor cell adhesion to activated endothelium, with a predominant seven-domain form identified.
Area of Science:
- Molecular Biology
- Immunology
- Cell Biology
Background:
- Vascular Cell Adhesion Molecule 1 (VCAM-1) is an inducible protein on vascular endothelium.
- VCAM-1 mediates leukocyte and tumor cell adhesion via integrin VLA4.
- Human VCAM-1 has six immunoglobulin-like domains, with a seventh identified via alternative splicing.
Purpose of the Study:
- To clone and determine the nucleotide sequence of rat VCAM-1.
- To investigate the structure of VCAM-1 in a different species.
Main Methods:
- Cloning of rat VCAM-1 cDNA.
- Nucleotide sequencing.
Main Results:
- The nucleotide sequence of rat VCAM-1 was successfully obtained.
- Comparison with human VCAM-1 may reveal conserved and divergent structural features.
Conclusions:
- The study provides the genetic sequence for rat VCAM-1.
- This data facilitates comparative studies of VCAM-1 structure and function across species.