Related Experiment Videos
A prominent epitope on GABAA receptors is recognized by two different monoclonal antibodies
M Ewert1, A L de Blas, H Möhler
1Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, F.R.G..
Abstract:
The monoclonal antibody 62-3G1 raised against the GABAA/benzodiazepine receptor complex was tested for its subunit selectivity using recombinantly expressed GABAA receptor subunits. The antibody bound selectively to beta 2 and beta 3 but not beta 1 nor any other GABAA receptor subunit. Using enzyme-linked immunosorbent assay, the epitope on beta 2 and beta 3 subunits was determined to be residues 1-3. MAb bd 17, which displays identical subunit selectivity as mAb 62-3G1, was seen to bind to the same epitope. These results resolve the subunit selectivity of mAbs 62-3G1 and bd 17 and reveal the identity and localization of a prominent immunogenic epitope on the GABAA/benzodiazepine receptor.