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Related Experiment Videos

Macromolecular transport across endothelial monolayers.

R C Schaeffer1, M S Bitrick, W C Holberg

  • 1Benjamin W. Zweifach Microcirculation Laboratories, Veterans Administration Medical Center, Tucson, AZ 85723.

International Journal of Microcirculation, Clinical and Experimental
|May 11, 1992
PubMed
Summary

We discovered that endothelial cell layers on filters exhibit convective solute transport, known as solute drag. This effect is influenced by pore size and cell layer coverage, highlighting the barrier properties of confluent endothelial monolayers.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Understanding macromolecular transport across biological barriers is crucial for drug delivery and physiological studies.
  • Polycarbonate (PC) filters offer tunable pore sizes for investigating transport phenomena.
  • Endothelial cell monolayers serve as in vitro models for vascular barriers.

Purpose of the Study:

  • To characterize the size-selective transport of macromolecules across PC filters and EC monolayers.
  • To investigate the influence of pore size, hydrostatic pressure, and EC monolayer confluence on solute transport.
  • To elucidate the mechanisms governing macromolecular transport, including diffusion and convective solute drag.

Main Methods:

  • Utilized PC filters with varying pore radii (400–15,000 Å).

Related Experiment Videos

  • Employed FITC-HES (16–100 Å) and MDPF-BSA (35.5 Å) as model solutes.
  • Assessed transport under zero and applied hydrostatic pressure across filter-EC monolayer sandwiches.
  • Quantified monolayer permeability and cell coverage.

    • Main Results:

    • Observed significant convective solute transport (solute drag) across filter-EC monolayer sandwiches, particularly with larger pore sizes.
    • Solute drag was enhanced by positive hydrostatic pressure and diminished by negative pressure.
    • Increasing EC monolayer confluence progressively attenuated solute drag, correlating with increased cell coverage.
    • Low permeability monolayers exhibited restricted diffusion distinct from capillary transport, demonstrating barrier function.

    Conclusions:

    • Solute transport across EC monolayers is a combination of diffusion and convective solute drag.
    • The degree of solute drag is directly dependent on the confluence of the EC monolayer.
    • High-confluence EC monolayers create significant barrier effects in vitro, impacting macromolecular transport.