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Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme
A M Pyle1, F L Murphy, T R Cech
1Howard Hughes Medical Institute, University of Colorado, Boulder 80309.
Nature
|July 9, 1992
Abstract:
In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contributes to the stability of this arrangement and propose that it accepts a hydrogen bond from a specific 2'-OH in P1. Such base-backbone tertiary interactions may be generally important to the organization of RNA tertiary structure.
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