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Related Experiment Videos

Visualization of histamine binding to nuclei.

P Bagavandoss1, M A Mitchell, J W Wilks

  • 1Cancer and Infectious Diseases Research, Upjohn Laboratories, Kalamazoo, Michigan 49001.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|May 1, 1992
PubMed
Summary

Histamine binds to ovarian cell membranes and nuclei. Antihistamines like pyrilamine block membrane binding, directing histamine to cell nuclei, suggesting novel nuclear roles.

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Area of Science:

  • Reproductive biology
  • Cell biology
  • Pharmacology

Background:

  • Histamine is a biogenic amine involved in various physiological processes.
  • Histamine receptors are known to be present on various cell types, including endothelial and ovarian cells.
  • The precise localization and function of histamine binding sites within ovarian cells are not fully understood.

Purpose of the Study:

  • To investigate the localization of histamine binding sites in cultured microvascular endothelial cells and ovarian tissue.
  • To determine the effect of specific histamine receptor antagonists on histamine binding patterns.

Main Methods:

  • Utilized fluorescein-albumin-histamine conjugate for visualizing histamine binding.
  • Examined binding patterns in cultured microvascular endothelial cells and glycol methacrylate embedded ovarian tissue sections.

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  • Assessed the impact of cimetidine and pyrilamine on histamine binding to plasma membranes and nuclei.

    • Main Results:

    • Histamine conjugate bound to plasma membranes and nuclei of luteal, endothelial, and ovarian stromal cells.
    • Cimetidine increased nuclear histamine binding while plasma membrane binding remained.
    • Pyrilamine completely inhibited plasma membrane binding, with histamine exclusively binding to nuclei of endothelial, germinal epithelial, granulosa, and stromal cells.
    • Nuclei of terminally differentiated luteal cells and oocytes did not show histamine binding.

    Conclusions:

    • Histamine exhibits distinct binding patterns to ovarian cells, localizing to both plasma membranes and nuclei.
    • Histamine receptor antagonists differentially affect histamine distribution, with pyrilamine specifically targeting nuclear binding sites.
    • The presence of nuclear histamine binding sites suggests potential novel intracellular functions of histamine within the ovary that warrant further investigation.