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Raw soybeans stimulate human pancreatic proteinase secretion
H Holm1, J E Reseland, L I Thorsen
1Institute for Nutrition Research, University of Oslo, Norway.
The Journal of Nutrition
|July 1, 1992
Summary
Raw soybeans stimulate pancreatic proteinase secretion in humans, despite inhibiting existing enzymes. This response, mediated by nervous regulation, ensures digestive capacity is maintained.
Area of Science:
- Gastroenterology
- Human Physiology
- Nutritional Science
Background:
- Raw soybeans contain Kunitz trypsin inhibitor, which affects digestive enzyme activity.
- Pancreatic proteinase secretion is crucial for nutrient digestion.
- Previous studies indicated no cholecystokinin (CCK) elevation with soybean ingestion.
Purpose of the Study:
- To investigate the effect of intraduodenal raw soybean instillation on human pancreatic proteinase secretion.
- To explore the role of Kunitz trypsin inhibitor in this response.
- To understand the regulatory mechanisms involved.
Main Methods:
- Intraduodenal instillation of raw soybeans and saline in human subjects.
- Measurement of immunoreactive trypsin and chymotrypsin using rocket immunoelectrophoresis.
- Assessment of amylase secretion.
- Analysis of plasma cholecystokinin (CCK) concentrations.
Main Results:
- Raw soybeans significantly stimulated pancreatic proteinase secretion, with immunoreactive trypsin and chymotrypsin increasing 2-4 fold.
- Soybeans reduced existing chymotrypsin activity and tryptic activity by 50%.
- The stimulation persisted and was not correlated with CCK levels, suggesting nervous regulation.
Conclusions:
- Kunitz trypsin inhibitor in raw soybeans triggers a nervous-mediated pancreatic proteinase secretion.
- This response includes the release of inhibitor-resistant trypsin, restoring digestive capacity.
- Nervous, potentially cholinergic, pathways regulate this adaptive digestive mechanism.