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Identification of Brucella spp. by using the polymerase chain reaction
1Rijkszuivelstation, Centrum voor Landbouwkundig Onderzoek, Melle, Belgium.
Applied and Environmental Microbiology
|June 1, 1992
Abstract:
The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens.