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Related Experiment Videos

CD16 on human gamma delta T lymphocytes: expression, function, and specificity for mouse IgG isotypes.

E Braakman1, J G van de Winkel, B A van Krimpen

  • 1Department of Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.

Cellular Immunology
|August 1, 1992
PubMed
Summary

CD16, a receptor on human gamma delta T cells, mediates cytotoxic activity and antibody-dependent cellular cytotoxicity (ADCC). Its function is linked to CD3 pathways and shows specific binding to mouse IgG isotypes.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD16 (Fc gamma receptor III) is a key molecule in immune responses.
  • Gamma delta T cells are a distinct T cell subset with unique immune functions.

Purpose of the Study:

  • To investigate the expression, function, and antibody-isotype specificity of CD16 on human gamma delta T cells.
  • To compare CD16 function on gamma delta T cells with that on natural killer (NK) cells.

Main Methods:

  • Flow cytometry for CD16 expression analysis.
  • Antibody-dependent cellular cytotoxicity (ADCC) assays.
  • EA rosette assays using mouse IgG isotype switch variants.

Main Results:

  • CD16 is expressed on the majority of peripheral blood gamma delta T cells and a subset of clones.

Related Experiment Videos

  • CD16 on gamma delta T cells mediates cytotoxic activity and ADCC, similar to NK cells.
  • CD16 exhibits highest affinity for mouse IgG2a, intermediate for IgG2b, and no binding for IgG1.

    • Conclusions:

    • CD16 is a functional receptor on human gamma delta T cells, contributing to cytotoxic responses.
    • A functional linkage exists between CD16 and CD3 signaling pathways in gamma delta T cells.
    • The mouse IgG-isotype specificity of CD16 on gamma delta T cells mirrors that on NK cells.