Immunoreactivity, epitope mapping and protection studies with anti-conotoxin GI sera and various conotoxins

¹Department of Toxinology, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702-5011.

Toxicon : Official Journal of the International Society on Toxinology

|April 1, 1992

Abstract:

Goat or rabbit anti-conotoxin GI sera recognized native or dithiothreitol-reduced alpha-conotoxins GI, MI and SI in an enzyme-linked immunosorbent assay (ELISA). Native mu-conotoxin GIIIA or omega-conotoxin GVIA did not react with either anti-conotoxin GI serum in the assay. The goat anti-conotoxin GI serum neutralized 2.5 LD50S of alpha-conotoxins GI or MI in mouse lethal assays, while the rabbit antiserum had little protective capabilities. Epitope mapping of synthesized conotoxin peptide fragments revealed that both anti-conotoxin GI sera recognized linear sequences from five different alpha-conotoxins: GI, GIA, GII, MI and SI. The CCNPAC sequence was optimally recognized by both antisera.

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Abstract:

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