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Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
Published on: September 12, 2016
Salmonella enteritidis-specific monoclonal antibodies
A W Lin1, R A Goldsby, G H Snoeyenbos
1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003.
Avian Diseases
|April 1, 1992
Summary
Researchers developed three monoclonal antibodies (MAbs) that specifically detect Salmonella enteritidis. These MAbs offer potential for improved diagnostic tools to identify this important foodborne pathogen.
Area of Science:
- Immunology
- Microbiology
- Pathogen Detection
Background:
- Salmonella enteritidis is a significant foodborne pathogen.
- Specific diagnostic reagents are crucial for identifying S. enteritidis.
- Monoclonal antibodies (MAbs) offer high specificity for target antigens.
Purpose of the Study:
- To derive and characterize monoclonal antibodies (MAbs) specific for Salmonella enteritidis.
- To evaluate the utility of these MAbs for pathogen detection.
Main Methods:
- Immunization of BALB/c mice with ultraviolet-killed S. enteritidis.
- Hybridoma technology for MAb production.
- Testing MAb reactivity against various Salmonella species and field isolates.
Main Results:
- Three MAbs (1053, 1110, 1170) specific for S. enteritidis were successfully derived.
- These MAbs reacted with multiple S. enteritidis field isolates, including phage type 4.
- No cross-reactivity was observed with a diverse range of other Salmonella species.
- The target determinant is heat-labile, with at least two MAbs recognizing the same epitope.
Conclusions:
- The developed MAbs are highly specific for Salmonella enteritidis.
- These MAbs represent promising tools for the accurate diagnosis and detection of S. enteritidis infections.
- Further characterization of the heat-labile determinant is warranted.
