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The cDNA cloning, nucleotide sequence and expression of an intracellular protein tyrosine phosphatase from mouse
1Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health Research Triangle Park, North Carolina 27709.
Abstract:
The PTP-1 cDNA encoding an intracellular protein tyrosine phosphatase (PTPase) was isolated and sequenced from a mouse testis cDNA library. This PTP-1 cDNA was found to contain an open reading frame of 1,296 nucleotides as well as 5' (83 nucleotides) and 3' (289 nucleotides) non-coding regions. The deduced sequence of 432 amino acids of mouse PTPase-1 exhibited 93% and 83% identity to that of rat PTPase-1 and human PTPase-1B, respectively. Thus, this PTP-1 is a mouse homologue of human PTP-1B and rat PTP-1. Northern blot analysis indicated that PTP-1 mRNAs were most abundant in testis, and were detected in sizes of 4.4 Kb, 2.4 Kb and 2.2 Kb, 2.0 Kb. The PTP-1 transcripts of 4.4 Kb and 2.0 Kb, but not 2.4 Kb and 2.2 Kb, were also present in kidney, spleen, muscle, liver, heart and brain. Genomic blot analysis showed that a single copy of the PTP-1 gene is contained in the mouse genome and that introns are present in mammalian PTP-1 genes.