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32P-postlabelling analysis of DNA from human tissues
M N Routledge1, R C Garner, D Jenkins
1Jack Birch Unit for Environmental Carcinogenesis, Department of Biology, University of York, Heslington, UK.
Mutation Research
|July 1, 1992
Abstract:
DNA extracted from human lung, bladder, liver, pancreas, cervix and breast tissue samples taken at autopsy (37 sample sets) was analysed by the nuclease P1 enhancement modification of the 32P-postlabelling assay for levels of aromatic carcinogen DNA adducts. Results were combined with those from a previous study for statistical analysis of 56 sample sets (32 male+24 female). A strong trend was seen for increased adduct levels in the lung DNA of smokers and a weak association for the bladder DNA of smokers compared to non-smokers. Aromatic adducts were also detected in other tissues.