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T cell receptor V beta repertoire of double-negative alpha/beta T cells in patients with systemic sclerosis
A Sakamoto1, T Sumida, T Maeda
1Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Arthritis and Rheumatism
|August 1, 1992
Objective:
To analyze the T cell receptor V beta gene on double-negative (DN) alpha/beta T cells, which are increased in number, on peripheral blood lymphocytes (PBL) from patients with systemic sclerosis (SSc).
Methods:
The DN alpha/beta T cells were sorted by flow cytometry from PBL obtained from 3 patients with SSc. The V beta repertoire was analyzed by polymerase chain reaction.
Results:
Only 1 or 2 V beta genes (V beta 5/7, 5, or 17) were predominantly expressed on DN alpha/beta T cells from these 3 patients.
Conclusion:
The V beta repertoire on DN alpha/beta T cells in PBL from patients with SSc is rather restricted.