Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple Rh messenger RNA isoforms are produced by alternative splicing.

C Le Van Kim1, B Chérif-Zahar, V Raynal

  • 1Unité INSERM U76, Institut National de Transfusion Sanguine, Paris, France.

Blood
|August 15, 1992
PubMed
Summary

Researchers identified novel Rh-related cDNAs in human bone marrow, revealing potential Rh protein isoforms with unique structural features and possible links to Rh antigenic variants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TSPO ligands stimulate ZnPPIX transport and ROS accumulation leading to the inhibition of P. falciparum growth in human blood.

Scientific reports·2016
Same author

Role of Lu/BCAM glycoproteins in red cell diseases.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2010
Same author

Red cell and endothelial Lu/BCAM beyond sickle cell disease.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2008
Same author

Role of Lu/BCAM in abnormal adhesion of sickle red blood cells to vascular endothelium.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2008
Same author

Noninvasive fetal RHD genotyping from maternal plasma. Use of a new developed Free DNA Fetal Kit RhD.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2008
Same author

Hydrophobic cluster analysis and modeling of the human Rh protein three-dimensional structures.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2006

Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • The Rh blood group system is crucial in transfusion medicine and understanding its genetic basis is important.
  • Previous studies identified a single Rh polypeptide, but the full complexity of Rh protein expression remained unclear.

Purpose of the Study:

  • To isolate and characterize novel Rh-related cDNAs from human bone marrow.
  • To investigate the structural properties and potential diversity of Rh protein isoforms.

Main Methods:

  • cDNA library screening of human bone marrow.
  • Polymerase chain reaction (PCR) amplification of bone marrow and erythroblast mRNAs.
  • Analysis of cDNA sequences for structural variations and potential protein modifications.

Related Experiment Videos

Main Results:

  • Three novel Rh-related cDNAs were identified, suggesting a family of Rh protein isoforms.
  • These isoforms exhibit significant structural differences, including peptide deletions and altered membrane topology.
  • Evidence suggests these variants arise from alternative splicing of the same Rh gene and are present in erythroid cells and reticulocytes.

Conclusions:

  • The Rh gene locus likely produces multiple protein species, potentially accounting for different Rh antigenic variants.
  • These findings expand our understanding of Rh blood group system complexity at the molecular level.