Evidence for a substance P containing subpopulation in the primate suprachiasmatic nucleus

Immunohistochemical detection of substance P (SP) in the suprachiasmatic nucleus (SCN) of the Old World monkey, Macaca fascicularis, was performed using two different rabbit polyclonal antisera. Immunostaining revealed a large population of neurons located in the dorsal subdivision of the nucleus identified by Nissl stain. This neuronal group represents the only cluster of SP-like immunoreactive (SP-IR) perikarya observed within the hypothalamus. In contrast with our present finding in the macaque, earlier studies only reported a few scattered SP-IR neurons in the SCN of other mammalian species. In agreement with previous descriptions of neuropeptides in the SCN, the topographical distribution of SP-IR neurons in the monkey confirms that cellular segregation is a significant feature of the mammalian SCN. This particular peptidergic subpopulation may represent a characteristic of the monkey circadian pacemaker. Together with other anatomical data previously reported in monkey and man, this finding also relates to the anatomical evolution of the circadian system from non-primates to humans. Although convincing data support the implication of SP in cyclic neuroendocrine regulations, the role of this tachykinin in circadian rhythmicity remains to be elucidated.